New & Simplified DNA Methylation Profiling Method: Bio CAP (Biotinylated CxxC Affinity Purification)

When a portrait artist is drawing a subject, it is often helpful to view the work in progress, as a reflection in a mirror. Somehow it re-attunes the mind’s eye to better assess the quality of the image. In a similar way, recently developed methods allow scientists to reflect on disease, or developmental states, by isolating NON-methylated DNA for profiling. Illinngworth et al., cleverly developed the CAP method in 2008. It produces profiles of non-methylated DNA using a zinc finger CxxxC domain which specifically recognizes non-methylated CpGs. The downsides to this method are that it requires a high-resolution chromatographic system, a large amount of the recombinant CxxC module, large amounts of input DNA (to account for elution and handling steps), … Continue reading →