In this webinar, we discuss simple and quick enzymatic methods for global, as well as sequence-specific, determination of both DNA modifications throughout various genomes (1). T4 phage beta-glucosyltransferase has emerged as the enzyme of choice for genome-wide 5-hmC quantifications. Similarly, using MspI sensitivity to glucosylated 5-hydroxymethylcytosine, we have established a scheme to interrogate differential methylation status at specific loci (2). In this webinar, various enzymatic techniques and their advantages will be discussed. Refereence: 1. Terragni, J., Bitinaite, J., Zheng, Y and Pradhan, S. (2012) Biochemical characterization of recombinant beta-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes- unpublished 2. Kinney SM, Chin HG, Vaisvila R, Bitinaite J, Zheng Y, Estève PO, Feng S, Stroud H, Jacobsen SE, Pradhan S. (2011) Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes. J Biol Chem. 286(28):24685-93.