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Author (up) Blecher-Gonen, R.; Barnett-Itzhaki, Z.; Jaitin, D.; Amann-Zalcenstein, D.; Lara-Astiaso, D.; Amit, I.
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  Title High-throughput chromatin immunoprecipitation for genome-wide mapping of in vivo protein-DNA interactions and epigenomic states Type Journal Article
  Year 2013 Publication Nature Protocols Abbreviated Journal Nat Protoc  
  Volume 8 Issue 3 Pages 539-554  
  Keywords  
  Abstract Dynamic protein binding to DNA elements regulates genome function and cell fate. Although methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect of genomic research, they are laborious and costly, thereby limiting progress. Here we present a protocol for mapping in vivo protein-DNA interactions using a high-throughput chromatin immunoprecipitation (HT-ChIP) approach. By using paramagnetic beads, we streamline the entire ChIP and indexed library construction process: sample transfer and loss is minimized and the need for manually labor-intensive procedures such as washes, gel extraction and DNA precipitation is eliminated. All of this allows for fully automated, cost effective and highly sensitive 96-well ChIP sequencing (ChIP-seq). Sample preparation takes 3 d from cultured cells to pooled libraries. Compared with previous methods, HT-ChIP is more suitable for large-scale in vivo studies, specifically those measuring the dynamics of a large number of different chromatin modifications/transcription factors or multiple perturbations.  
  Address Department of Immunology, Weizmann Institute of Science, Rehovot, Israel  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1750-2799 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:23429716 Approved no  
  Call Number @ @ Serial 38049  
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