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Author (up) Blecher-Gonen, R.; Barnett-Itzhaki, Z.; Jaitin, D.; Amann-Zalcenstein, D.; Lara-Astiaso, D.; Amit, I.
url  doi
  Title High-throughput chromatin immunoprecipitation for genome-wide mapping of in vivo protein-DNA interactions and epigenomic states Type Journal Article
  Year 2013 Publication Nature Protocols Abbreviated Journal Nat Protoc  
  Volume 8 Issue 3 Pages 539-554  
  Abstract Dynamic protein binding to DNA elements regulates genome function and cell fate. Although methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect of genomic research, they are laborious and costly, thereby limiting progress. Here we present a protocol for mapping in vivo protein-DNA interactions using a high-throughput chromatin immunoprecipitation (HT-ChIP) approach. By using paramagnetic beads, we streamline the entire ChIP and indexed library construction process: sample transfer and loss is minimized and the need for manually labor-intensive procedures such as washes, gel extraction and DNA precipitation is eliminated. All of this allows for fully automated, cost effective and highly sensitive 96-well ChIP sequencing (ChIP-seq). Sample preparation takes 3 d from cultured cells to pooled libraries. Compared with previous methods, HT-ChIP is more suitable for large-scale in vivo studies, specifically those measuring the dynamics of a large number of different chromatin modifications/transcription factors or multiple perturbations.  
  Address Department of Immunology, Weizmann Institute of Science, Rehovot, Israel  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1750-2799 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:23429716 Approved no  
  Call Number @ @ Serial 38049  
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