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Author  |
Zheng, Y.; Cohen-Karni, D.; Xu, D.; Chin, H.G.; Wilson, G.; Pradhan, S.; Roberts, R.J. |
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Title |
A unique family of Mrr-like modification-dependent restriction endonucleases |
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Journal Article |
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Year |
2010 |
Publication |
Nucleic Acids Research |
Abbreviated Journal |
Nucleic Acids Res |
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Volume |
38 |
Issue |
16 |
Pages |
5527-5534 |
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Keywords |
Amino Acid Sequence; DNA/chemistry/metabolism; DNA Restriction Enzymes/chemistry/classification/metabolism; Escherichia coli Proteins/chemistry/classification; Genome; Hela Cells; Humans; Molecular Sequence Data; Mycobacterium/enzymology; Sequence Alignment; Substrate Specificity |
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Abstract |
Mrr superfamily of homologous genes in microbial genomes restricts modified DNA in vivo. However, their biochemical properties in vitro have remained obscure. Here, we report the experimental characterization of MspJI, a remote homolog of Escherichia coli's Mrr and show it is a DNA modification-dependent restriction endonuclease. Our results suggest MspJI recognizes (m)CNNR (R = G/A) sites and cleaves DNA at fixed distances (N(12)/N(16)) away from the modified cytosine at the 3' side (or N(9)/N(13) from R). Besides 5-methylcytosine, MspJI also recognizes 5-hydroxymethylcytosine but is blocked by 5-glucosylhydroxymethylcytosine. Several other close homologs of MspJI show similar modification-dependent endonuclease activity and display substrate preferences different from MspJI. A unique feature of these modification-dependent enzymes is that they are able to extract small DNA fragments containing modified sites on genomic DNA, for example approximately 32 bp around symmetrically methylated CG sites and approximately 31 bp around methylated CNG sites. The digested fragments can be directly selected for high-throughput sequencing to map the location of the modification on the genomic DNA. The MspJI enzyme family, with their different recognition specificities and cleavage properties, provides a basis on which many future methods can build to decode the epigenomes of different organisms. |
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Address |
New England BioLabs, Inc., 240 County Road, Ipswich, MA, 01938, USA. zhengy@neb.com |
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English |
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0305-1048 |
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Notes |
PMID:20444879 |
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Call Number |
pubs @ support @ |
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6886 |
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