TY  - JOUR
AU  - Saadeldin, I.M.
AU  - Choi, W.
AU  - Torre, B.R.
AU  - Kim, B.
AU  - Lee, B.
AU  - Jang, G.
PY  - 2012//
TI  - Embryonic Development and Implantation Related Gene Expression of Oocyte Reconstructed with Bovine Trophoblast Cells
T2  - J Reprod Dev
JO  - The Journal of Reproduction and Development
N2  - The temporal progressive increase of interferon tau (IFNtau) secretion from the bovine trophoblast is a major embryonic signal of establishing pregnancy. Here, we cultured and isolated bovine trophoblast cells (BTs) from IVM/IVF oocytes and in vitro produced blastocysts, used them, for the first time, as donor cells for nuclear transfer and compared them with adult fibroblasts (AFs) as donor cells. BTs were reprogrammed in enucleated oocytes to blastocysts with similar efficiency to AFs (14.5% and 15.6% respectively, P</=0.05). The levels of IFNtau, CDX2 and OCT4 expression in IVF-, BT- and AF-derived blastocysts were analyzed using reverse transcription polymerase chain reaction and reverse transcription quantitative polymerase chain reaction (RT-PCR and RT-qPCR). IVF-produced embryos were used as reference to analyze the linear progressive expression of IFNtau through mid, expanded and hatching blastocysts. RT-PCR and RT-qPCR studies showed that IFNtau expression was higher in BT-derived blastocysts than IVF- and AF-derived blastocysts. Both IVF- and BT-derived blastocysts showed a progressive increase in IFNtau expression as blastocyst development advanced when it compared with AF-derived blastocysts. OCT4 was inversely related with IFNtau expression, while CDX2 was found to be directly related with IFNtau temporal expression. Persistence of high expression of IFNtau and CDX2 was found to be higher in BT-derived embryos than in IVF- or AF-derived embryos. In conclusion, using BTs expressing IFNtau as donor cells for bovine NT could be a useful tool for understanding the IFNtau genetics and epigenetics.
SN  - 0916-8818
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22522228
N1  - PMID:22522228
ID  - Saadeldin_etal2012
ER  -